Akt (Rac PKa, PKBa)

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once\nphosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence (paraformaldehyde): 1:50-1:500\nELISA: 1:1000-1:5000\nWestern Blot: 1:250-1:1000\nImmunohistochemistry (Formalin, paraffin): 1:250-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1124-03A

Size

100ul

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Ch/Bird

Form

Supplied as liquid in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%. No stabilizing proteins added.

P Type

Pab

Purity

Serum

References

1. Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin\nresponses. J. Cell Science 114:2903-2910. 2. Alessi, D. R. (2001). Discovery of PDK1, one of the\nmissing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14.

Additional Info

Recognizes AKT from human tissues. Species sequence homology: rat, mouse and chicken AKT.