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Akt Substrate, phosphorylated (RXRXXS/T)

Akt Substrate, phosphorylated (RXRXXS/T)

Cat no: A1124-02K

Supplier: United States Biological
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An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2).Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis and transcriptional regulation (3-5). While a number of Akt substrates including GSK-3, Bad and caspase-9 are known, many important substrates await discovery. Akt phosphorylates substrates only at serine/threonine in a conserved motif characterized by arginine at positions -5 and -3 (6).Phospho-Akt substrate-specific antibodies are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Wesetrn Blot. Other applications have not been tested. Recommended Dilutions: Western blot:1:1000 Immunoprecipitation :1:50 Immunohistochemistry (Paraffin): 1:800 Unmasking buffer/Antibody diluent Citrate/ ELISA: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A1124-02K
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1.Montminy, M. (1997) Annu Rev Biochem 66, 807-22.Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.Jiang, B.H. et al. (2000) Proc Natl Acad Sci U S A 97, 1749-53.Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.
Additional info: Recognizes peptides and proteins containing phospho-serine/threonine preceded by arginine at positions -5 and -3, in a manner largely independent of the surrounding amino acid sequence. Some cross-reactivity is observed for peptides that contain phospho-serine/threonine preceded by arginine at position -3 . No cross-reactivity is observed with the corresponding nonphosphorylated sequences or with other phospho-serine/threonine-containing motifs. By ELISA, the antibody recognizes a wide range of peptides with phospho-threonine or Phospho-serine with arginine at -3 and -5 position. Species crossreactivity: All species.

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