Akt Substrate, Phosphorylated (Ser/Thr) (Rac PKa, PKBa)

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2).\nAkt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis and transcriptional regulation (3-5). While a number of Akt substrates including GSK-3, Bad and caspase-9 are known, many important substrates await discovery. Akt phosphory- lates substrates only at serine/threonine in a conserved motif characterized by arginine at positions -5 and -3 (6).\nPhospho-Akt substrate-specific antibodies are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg- directed kinases, as well as for high throughput kinase drug discovery.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA-Peptide: 1:500\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:300 using citrate as an unmasking buffer.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1124-50

Size

100ul

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Montminy, M. et al. (1997) Annu. Rev. Biochem. 66, 807-822. 2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol. 200, 62-82. 3. Marte, B.M. and Downward, J. (1997) Trends Biochem. Sci. 22, 355-358. 4. Jiang, B.H. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 1749-1753. 5. Scheid, M.P. and Woodgett, J.R. (2000) Curr. Biol. 10, R191-R194. 6. Alessi, D.R. et al. (1996) FEBS Lett. 399, 333-338.

Additional Info

Recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some crossreactivity for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. Does not crossreact with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. Species Crossreactivity: All