Akt1, active, Recombinant, Human (RAC-alpha Serine/Threonine-protein Kinase, Protein Kinase B, PKB, Protein Kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha)

Akt1 or Protein Kinase B alpha (PKBa) is a serine/threonine kinase that belongs to the Akt family. Akt1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt1 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2 (possibly ILK) (3). Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (4). In addition, the cyclin dependent kinase inhibitors are substrates of Akt which when phosphorylated relinquish their inhibitory influence on cell cycle progression. Akt also mediates many of the stimulatory effects of insulin on glucose metabolism through deactivation of glycogen synthase kinase, activation of phosphofructo-kinase, and modulation of glucose transporter activity. Akt1 has been shown to play a role in human cancers (4). It is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (5). Akt1 plays an important role in cancer cell survival and proliferation thereby contributing to cancer progression.\n\nSource: \nRecombinant corresponding to full length human Akt1 containing N-terminal GST tag, expressed by baculovirus in Sf9 insect cells.\n\nMolecular Weight: \n~85kD\n\nBiological Activity:\n124nmol phosphate incorporated into Akt substrate peptide/min/mg protein at 30 degrees C for 15 minutes using a final concentration of 50uM ATP and total of 0.83uCi/ul P-32.\n\nSpecific Activity:\n124nmol/min/mg\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1125-06K

Size

5ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Coffer PJ, Jin J, Woodgett JR. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13. 2. Anderson KE, Coadwell J, Hawkins PT. Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91. 3. Persad S, Attwell S, Gray V, Mawji N, Deng JT, Leung D, Yan J, Sanghera J, Dedhar S. Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343. J Biol Chem. 2001 Jul 20; 276(29): 27462-9. 4. Sen P, Mukherjee S, Ray D, Raha S. Involvement of the Akt/PKB signaling pathway with disease processes. Mol Cell Biochem. 2003 Nov; 253(1-2): 241-6. 5.Nicholson KM, Anderson NG. The protein kinase B/Akt signalling pathway in human malignancy. Cell Signal. 2002 May; 14(5): 381-95.

Alternative Names

EC=2.7.11.1