Akt1, Human, phosphorylated, active, Western Blot Positive Control (v-akt Mouse Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) Recombinant

Putative human homologs of the protooncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned. These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt. RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the C-terminus. The N-terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins. The PH domain (aa1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides. The kinase domain is located between residues 148-411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: Load ~10 ul/lane.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6-12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1125-01

Size

50ul

Applications

WB

Form

Supplied in SDS-PAGE sample buffer (reduced). This preparation of AKT1 is phosphorylated but not active in sample buffer.

Purity

Highly purified ((same/more than) 90%).

References

1. Konishi, et al., BBRC 205: 817-825 (1994). 2. Coffer & Woodgett, Eur. J. Biochem. 201: 475-481 (1991). 3. Jones, et al., PNAS USA 88: 4171-4175 (1991). 4. Marte, B.M., Downward, J., TIBS 22: 355 (1997).

Additional Info

This preparation was active (Specific Activity: 300-1800u/mg). AKT1 was activated with MAPKAP kinase 2 and PDK1 and repurified.