Akt1, PH Domain, Serine-Threonine Phosphorylation (Rac PKa, PKBa)

Supplier:

United States Biological

Cat no: A1125-04

Akt1, PH Domain, Serine-Threonine Phosphorylation (Rac PKa, PKBa)

Applications:\nSuitable for use in Flow Cytometry, Western Blot, Immunocytochemistry, Immunoprecipitation Kinase and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot Analysis: 0.1-1ug/ml detects Akt (60kD) in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1125-04 (0.5 and 1.0ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. \nImmunoprecipitation: 4ug immunoprecipitates Akt from 500ug of L6 RIPA lysate. \nImmunoprecipitation Kinase: 4ug immunoprecipitates active Akt from 700ug of L6 cells stimulated with 15nM IGF-1 for 2-5 min. \nOptimal dilutions to be determined by the researcher.\n\nPositive Antigen Control Included: \nA0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. \n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-14C: IgG, Fc (HRP) Pab Gt xMo\nI1904-19C: IgG, F(ab

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SPECIFICATIONS

Catalog Number

A1125-04

Size

100ug

Applications

FC, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Akt1/PKBa > Akt2/PKBb (30%) > Akt3/PKBg (weak to none) based on Western blot analysis using 100ng recombinant fusion protein for each isoform. \nSpecies Crossreactivity: mouse and rat.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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