Akt1, phosphorylated (Ser473) (RAC-alpha Serine/Threonine-protein Kinase, Protein Kinase B, PKB, Protein Kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha)

Supplier:

United States Biological

Cat no: A1125-13G

Akt1, phosphorylated (Ser473) (RAC-alpha Serine/Threonine-protein Kinase, Protein Kinase B, PKB, Protein Kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha)

Akt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis, although the pathways involved have not been identified. Akt is activated by phospholipid binding and phosphorylation within the activation loop at Thr308 and within the C-terminus at Ser473.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-4ug/ml\nImmunoprecipitation: 10-20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1125-13G

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes mouse Akt when phosphorylated at Ser473. Species Crossreactivity: Human and rat.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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