Home  >  Products  >  Akt1, phosphorylated (Ser473) (RAC-alpha Serine/Threonine-protein Kinase, Protein Kinase B, PKB, Proto-oncogene c-Akt, RAC-PK-alpha, PKB, RAC)
Akt1, phosphorylated (Ser473) (RAC-alpha Serine/Threonine-protein Kinase, Protein Kinase B, PKB, Proto-oncogene c-Akt, RAC-PK-alpha, PKB, RAC)

Akt1, phosphorylated (Ser473) (RAC-alpha Serine/Threonine-protein Kinase, Protein Kinase B, PKB, Proto-oncogene c-Akt, RAC-PK-alpha, PKB, RAC)

Cat no: A1125-12Q

Supplier: United States Biological
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PKB alpha (AKT1) is a member of the PKB family, which is responsive to the cell activation. The anti-PKB aplha antibodies were raised with a peptide based on sequence of human PKB C-terminal and affinity purified with the same peptide on agarose. The affinity purified antibodies were useful for detection the phosphosrylated form of AKT from the cell lysates. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1,000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A1125-12Q
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human Akt1 when phosphorylated at serine 473. Species Crossreactivity: mouse, rat.

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