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Akt1, phosphorylated (Ser473) (Rac PKa, PKBa)

Akt1, phosphorylated (Ser473) (Rac PKa, PKBa)

Cat no: A1125-04C

Supplier: United States Biological
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Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. Akt/PKB phosphorylation is critical for it activity. The major phosphorylation sites required for Akt activation has been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2. Positive Control: PDGF treated NIH 3T3 Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A1125-04C
Reactivities: Human, Mouse, Chicken/Bird
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Form: PBS containing 0.2% gelatin and 0.05% sodium azide.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein G affinity chromatography.
Alternative names: v-akt Murine Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein Kinase B, RAC, RAC-ALPHA, RAC-alpha Serine/threonine-protein Kinase, RAC-PK-alpha
References: 1. Alessi DR., et al. EMBO J. 15: 6541-6551 (1996). 2. Ferring P., et al. Oncogene 18: 6129-6134 (1999). 3. Kandel ES, et al. Exp. Cell Res. 253: 210-219 (1999). 4. Cantley LC, et al. Proc Natl Acad Sci, USA, 96: 420-4245 (1999). 5. Franke TF, et al. Cell 88: 435-437 (1997).
Additional info: Detects specifically phosphorylated form of Akt1 expression in PDGF treated cells. Species Crossreactivity: Human, Mouse Predicted to crossreact with Frog and Chicken.

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