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Akt1, phosphorylated (Ser473) (Rac, PKa, PKBa, CAKT, Murine Thymoma Viral (vakt) Oncogene Homolog 1)

Akt1, phosphorylated (Ser473) (Rac, PKa, PKBa, CAKT, Murine Thymoma Viral (vakt) Oncogene Homolog 1)

Cat no: A1125-04E

Supplier: United States Biological
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Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:10,000 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: A1125-04E
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Burgering, B.M. and Coffer, P.J., Nature 376: 599
Additional info: Recognizes human Akt1 phosphorylated at serine 473. Species Crossreactivity: Mouse.

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