Akt1, PKBa, phosphorylated (Ser473) (Alexa Fluor(R)488)

Supplier:

United States Biological

Cat no: A1125-03B

Akt1, PKBa, phosphorylated (Ser473) (Alexa Fluor(R)488)

Applications: \nSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nQuality Control Testing\nImmunoblot Analysis: 2ug/ml of this lot detected phosphorylated Akt1/PKBa in RIPA lysates from mouse NIH-3T3 cells. Immunocytochemistry: 2ug/ml of this lot showed positive immunostaining for phosphorylated Akt1/PKBa in 3T3 cells treated with PDGF (100ng/mL, 20min.). Flow Cytometry: 0.2microg of this lot detected Akt1/PKBa in fixed and permeabilized 3T3, L6, and A431 cells (see data, page two).

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SPECIFICATIONS

Catalog Number

A1125-03B

Size

50ug

Applications

FC, ICC, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, 1% BSA, 0.05% Tween(R)-20, 0.05% sodium azide. Liquid at 4 degrees C.

P Type

Mab

Purity

Purified

Isotype

IgG1

Additional Info

Recognizes phosphorylatedAkt1/PKBa at Ser473, Mr 60kDa. This lot of antibody does not cross-react with Akt1/PKBa dephosphorylated at Ser473.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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