AKT1 (RAC-alpha Serine/Threonine-protein Kinase, Protein Kinase B, PKB, Proto-oncogene c-Akt, RAC-PK-alpha, PKB, RAC)

AKT1, initially identified as the cellular homolog to the retro-viral oncogene v-AKT, is part of the phosphatidyl 3'-kinase (PI3K)-AKT signaling pathway that is activated by diverse cellular stimuli and regulates critical cellular functions such as cell growth, proliferation, and survival. Following phosphorylation of the second messenger PIP2 by PI3K, AKT1 translocates to the cell membrane where it is activated by phosphoinositide-dependent kinase (PDK) 1 and PDK2. The active AKT1 is then able to phosphorylate and activate its substrates, including those that are important for cell proliferation and survival such as TOR and the Bcl-2 homolog Bad. Negative regulation of the PI3K-AKT signaling pathway is mainly accomplished by the lipid phosphatase activity of PTEN which catalyzes the conversion of PIP3 to PIP2, thereby preventing the activation of AKT1. Inactivation of this gene often results in excessive AKT1 activity, often leading to the formation of malignant tumors.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman liver cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1125-05U

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bellacosa A, Testa JR, Staal SP, et al. A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region. Science 1991; 254:274-7. 2. Song G, Ouyang G, and Bao S. The activation of Akt/PKB signaling pathway and cell survival. J. Cell. Mol. Med. 2005; 9:59-71. 3. Alessi DR, Adnjelkovic M, Caudwell B, et al. Mechanism of protein kinase B by insulin and IGF-1. EMBO J. 1996; 15:6541-51. 4. Nave BT, Ouwens M, Withers DJ, et al. Mammalian target of rapamycin is a direct target for protein kinase B: Identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation. Biochem. J. 1999; 344:427-31. 5. Datta SR, Dudek H, Tao X, et al. Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 1997; 91:231-41. 6. Eng C. PTEN: One gene, many syndromes. Hum. Mutat. 2003; 22:183-8.

Additional Info

Recognizes human AKT1. Species Crossreactivity: mouse and rat.