Akt2, active, Recombinant, Human (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, Protein Kinase Akt-2, Protein Kinase B beta, PKB beta)

Akt2 or Protein Kinase B b (PKBb) is a serine/threonine kinase that is a member of the Akt family. Akt2 like the other Akt members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3, 4, 5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2. Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (3).\n\nSource: \nRecombinant corresponding to full-length human Akt2 containing N-terminal His tag, expressed by baculovirus in Sf9 insect cells.\n\nMolecular Weight: \n~58kD\n\nBiological Activity:\n46nmol phosphate incorporated into Akt/SGK substrate (RPRAATF) per minute per mg protein at 30 degrees C for 15 minutes using a final concentration of 50uM ATP and total of 0.83uCi/ul P-32.\n\nSpecific Activity:\n46nmol/min/mg\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1125-26G

Size

5ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Coffer PJ, Jin J, Woodgett JR. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 ( Pt 1):1-13. 2. Anderson KE, Coadwell J, Stephens LR, Hawkins PT. Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684- 91. 3. Sen P, Mukherjee S, Ray D, Raha S. Involvement of the Akt/PKB signaling pathway with disease processes. Mol Cell Biochem. 2003 Nov; 253(1-2): 241-6.

Alternative Names

EC=2.7.11.1