Home  >  Products  >  Akt2 (AKT-2, Protein Kinase Akt2, Protein Kinase B beta, PKB beta, PKBBeta, PRKBB, Rac Protein Kinase beta, RAC-BETA, RAC-beta Serine/threonine Protein Kinase, RAC-PK-beta, v-AKT Murine Thymoma Viral Oncogene Homolog 2) (Azide Free)
Akt2 (AKT-2, Protein Kinase Akt2, Protein Kinase B beta, PKB beta, PKBBeta, PRKBB, Rac Protein Kinase beta, RAC-BETA, RAC-beta Serine/threonine Protein Kinase, RAC-PK-beta, v-AKT Murine Thymoma Viral Oncogene Homolog 2) (Azide Free)

Akt2 (AKT-2, Protein Kinase Akt2, Protein Kinase B beta, PKB beta, PKBBeta, PRKBB, Rac Protein Kinase beta, RAC-BETA, RAC-beta Serine/threonine Protein Kinase, RAC-PK-beta, v-AKT Murine Thymoma Viral Oncogene Homolog 2) (Azide Free)

Cat no: A1125-26J

Supplier: United States Biological
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Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1- 3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: 13000 dilution of SPC-148 was sufficient for detection of PKBB in 20ug of HeLa cell lysate by ECL immunoblot analysis. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A1125-26J
Reactivities: Human, Mouse, Rat, Bovine, Canine, Chicken/Bird, Guinea Pig, Non-Human Primate, Porcine, Sheep, Xenopus/Amphibian
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 400ul
Form: Supplied as a liquid in rabbit immunoglobulin in 0.1M Tris-acetate buffer, 0.1% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Downward J (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil DP, and Hemmings BA (2001). Biochem Sci. 26(11):657-64. 3. Nicholson KM, Anderson NG (2002). Cell Signal. 14(5):381-95. 4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.
Additional info: Recognizes human Akt2. Species Crossreactivity: mouse, rat, bovine, chicken, canine, guinea pig, hamster, monkey, porcine, sheep, xenopus.

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