The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of
such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In
mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha), Akt2 (PKB beta), and Akt3 (PKBg). Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat.
Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 50ug/ml
Immunohistochemistry: 25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.