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AKT2 (v-akt murine thymoma viral oncogene homolog 2, PKBB, PKBBETA, PKB beta, PRKBB, Protein kinase Akt-2, Protein kinase B beta, RAC-BETA, RAC-beta serine/threonine-protein kinase, RAC-PK-beta)

Cat no: A1125-25T

AKT2 (v-akt murine thymoma viral oncogene homolog 2, PKBB, PKBBETA, PKB beta, PRKBB, Protein kinase Akt-2, Protein kinase B beta, RAC-BETA, RAC-beta serine/threonine-protein kinase, RAC-PK-beta)

Akt2 (also designated protein kinase B beta or v-akt murine thymoma viral oncogene homolog 2 ), with 481-amino acid protein (about 53kDa), belongs to the AKT serine/threonine protein kinase family, which also includes Akt1 and Akt3. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. Among the members of AKT family, Akt2 is associated with the development of human cancers. Akt2 inhibits cisplatin-induced JNK/p38 and Bax activation through phosphorylation of ASK1 and thus, plays an important role in chemoresistance. Further, Akt2 plays a specific role in muscle differentiation.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000\nWestern Blot: 1:500-1:2000\nImmunofluorescence: 1:200-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1125-25T

Size

100ul

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum, Rat, NHP

Form

Supplied as liquid, 0.04% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2b

References

1. Am J Physiol Endocrinol Metab. 2004 Jul; 287(1):E8-E15, 2. Oncol Rep. 2004 Jan;11(1):25-32, 3. Cancer Res. 2003 Jan 1; 63(1):196-206.

Additional Info

Recognizes human AKT2 at 60kD. Species Crossreactivity: rat, monkey.

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