Akt3 (AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase)

Alternate Name: AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase\n\nDomain Information: \n\nGene Ontology Terms: ATP binding; protein amino acid phosphorylation; protein serine/threonine kinase activity; signal transduction; transferase activity\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: A 50-55kD band observed in Human Hepatoblastoma HepG2 lysates (calculated MW of 55.8kD according to NP_005456 and 54.0kD according to NP_859029). Recommended concentration: 1-3ug/ml.\n\n1ug/ml staining of HepG2lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See A1125-30F-P. Antibody

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SPECIFICATIONS

Catalog Number

A1125-30F

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR. , AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon., Clin Cancer Res. 2001 Aug;7(8):2475-9.

Additional Info

This antibody is expected to recognize an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Species Crossreactivity: human, mouse, rat.