Alanine Aminotransferase (ALT or SGPT) BioAssay(TM) Kit

Alanine aminotransferase (ALT), also called serum glutamic pyruvic transaminase (SGPT), is a transaminase (EC 2.6.1.2) or alanine transaminase. ALT is found in serum and in various body tissues, but is usually associated with the liver. It catalyzes the reaction:\n a-Ketoglutarate + Alanine ? Glutamate + Pyruvate\n\nIt is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In this ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ?-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (^max = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10mU per well.\n\nKit Components:\nA1210-22A: ALT Asay Buffer, 1x25ml\nA1210-22B: OxiRedTM (in DMSO), 1x200ul\nA1210-22C: ALT Enzyme Mix, 1x1vial. Lyophilized\nA1210-22D: ALT Substrate, 1x1vial. Lyophilized\nA1210-22E: Pyruvate Standard, 1x100ul. Supplied at a concentration of 100nmol/ul\nA1210-22F: ALT Positive Control, 1x1vial. Lyophilized\n\nStorage and Stability:\nStore all kit components at -20 degrees C, protected from light. Unopened kit components are stable for up to 6 months at -20 degrees C.

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