Alanine Transaminase (ALT) (HRP)

Alanine aminotransferase (ALT, GPT, Gutamate pyruvate transaminanse), catalyzes the transamination reaction. ALT requires pyridoxal-5-phosphate (PLP) as prosthetic group. ALT was previously known as serum glutamic pyruvic transaminase (SGPT). ALT is a cytoplasmic enzyme that catalyzes the transamination of alpha-ketoglutarate and L-alanine, forming glutamate and pyruvate. ALT is found in liver, kidney, brain and heart, as two isoforms, cytosolic ALT and mitochondrial ALT. These enzymes function in cellular nitrogen metabolism and also liver gluconeogenesis. The highest activities of ALT are found in hepatocytes and striated (skeletal and cardiac) muscle cells. Therefore, increased serum ALT activity can accompany hepatocellular injury or necrosis of striated muscle. With cell injury or death, ALT (a 'leakage' enzyme) escapes from the cytosol. Determination of ALT activity is a relatively sensitive indicator of hepatic damage in certain animal species and can help determine whether further diagnostic tests.\n\nApplications: \nSuitable for use in Western Blot, Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry and Conjugation. Other applications not tested.\n\nRecommended Dilution: \nELISA: 1:1000-1:3000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nAlso Available:\nA1210-05: Alanine Transaminase (ALT) Pab Sh xPo\nA1210-06: Alanine Transaminase (ALT) Pab Sh xPo\nA1210-15: Alanine Transaminase (ALT) (HRP) Pab Sh xPo\nA1210-20: A1210-15: Alanine Transaminase (ALT) (Biotin) Pab Sh xPo

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SPECIFICATIONS

Catalog Number

A1210-20

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Sheep

Reactivities

Bov, Prc

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate, before the addition of 40% glycerol. Label with Horseradish Peroxidase (HRP).

P Type

Pab

Purity

Puri?ed by delipidation, fractionation and ion exchange chromatography.

Isotype

IgG

Additional Info

Recognizes Alanine Transaminase. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified Alanine transaminase (ALT) (Porcine Heart). Species Crossreactivity: Reported to crossreact with bovine ALT in Western Blot.