Home  >  Products  >  ALAS1 (Aminolevulinate delta-Synthase 1, 5-Aminolevulinic Acid Synthase, Delta-ALA Synthetase)
ALAS1 (Aminolevulinate delta-Synthase 1, 5-Aminolevulinic Acid Synthase, Delta-ALA Synthetase)

ALAS1 (Aminolevulinate delta-Synthase 1, 5-Aminolevulinic Acid Synthase, Delta-ALA Synthetase)

Cat no: A1210-02D

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependent housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HepG2 Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A1210-02D
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein G affinity chromatography.
References: 1. Fujii,H., Takahashi,T., Matsumi,M., Kaku,R., Shimizu,H., Yokoyama,M., Ohmori,E., Yagi,T., Sadamori,H., Tanaka,N., Akagi,R. and Morita,K. Increased heme oxygenase-1 and decreased delta-aminolevulinate synthase expression in the liver of patients with acute liver failure. Int. J. Mol. Med. 14 (6), 1001-1005 (2004). 2. Bishop,D.F. Two different genes encode delta-aminolevulinate synthase in humans: nucleotide sequences of cDNAs for the housekeeping and erythroid genes Nucleic Acids Res. 18 (23), 7187-7188 (1990). 3. Bawden,M.J., Borthwick,I.A., Healy,H.M., Morris,C.P., May,B.K. and Elliott,W.H. Sequence of human 5-aminolevulinate synthase cDNA Nucleic Acids Res. 15 (20), 8563 (1987).
Additional info: Recognizes human ALAS1. Species sequence homology: mouse, rat, chimpanzee, canine, pufferfish, bovine, xenopus.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave