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Albumin, Human Serum (HSA)

Cat no: A1275-28B

Albumin, Human Serum (HSA)

Human serum albumin (HSA) consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5kD (1).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nX63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

A1275-28B

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, and15mM sodium azide, before the addition of glycerol to 40%.\n

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG1,k

References

1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.

Additional Info

Has specificity for native and denatured human serum albumin (HSA). Reacts strongly with native and denatured HSA. Strong reaction is seen in ELISA with native or denatured HSA directly coated onto the microtiterwell. In Western blotting after SDS-PAGE, this antibody reacts with native and denatured HSA in non-reduced form only. Epitope specificity differs from those of clones 6F11 and 6F12 but overlaps as determined by inhibition ELISA.\n

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