Aldehyde Dehydrogenase Activity Kit, BioAssay(TM) (ALDH)

The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis. Therefore the early detection of ALDH activity levels can be prognostic and guide the therapeutic strategies. USBioпїЅs Aldehyde Dehydrogenase Activity BioAssay(TM) Kit is a robust tool to quantify ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH to form the NADH which couples with the PicoProbe(TM) to generate a potent fluorescence (Ex/Em = 535/587). The ALDH fluorometric assay kit is 10X more sensitive than the ALDH colorimetric assay and can detect <0.05mU ALDH activity (based on our unit definition) in a variety of samples.\n\nSample Type:\nCell and Tissue culture supernatants, cell lysates, plasma and serum, as well as many other biological fluids. \n\nIntended Use:\nMeasuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses.\n\nSensitivity:\n<0.05mU\n\nSpecificity:\nMammalian\n\nKit Components:\nALDH Assay Buffer: 1x25ml\nAcetaldehyde: 1x500ul\nPicoProbe(TM) (in DMSO): 1x400ul\nALDH Substrate Mix (lyophilized) 1 vial\nALDH Positive Control (lyophilized) 1 vial\nNADH Standard (0.5umol, lyophilized) 1 vial\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts