Aldo-keto Reductase Family 1 Member C1, Recombinant, Human (AKR1C1, 20-alpha-hydroxysteroid Dehydrogenase, 20-alpha-HSD, 2-alpha-HSD, C9, Chlordecone Reductase Homolog HAKRC, Indanol Dehydrogenase, Dihydrodiol Dehydrogenase 1/2, DD1, DD1/DD2, DDH, DDH1, H

AKR1C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant AKR1C1 protein was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa1-270 of human AKR1C1 6x His tagged expressed in E. coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A1334-70L

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl pH 8.0, 1mM DTT, 20% glycerol.

Purity

~ 90% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. Zhang Y., et al. (2000) J. Mol. Endocrinol. 25:221-228 2. Zhang Y., et al. (2009) Mol Cell Endicrinol. 298(1-2):76-83

Alternative Names

EC=1.1.1.-, EC=1.1.1.112, EC=1.1.1.149, EC=1.3.1.20, Aldo-keto Reductase Family 1, Member C1 (Dihydrodiol Dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid Dehydrogenase)