Aldolase B

Aldolase B is one of three known aldolase isoenzymes, and is found in kidney and small adult intestine where it is associated with aldolases A or C. Aldolase B catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is involved in both glycolytic and gluconeogenic pathways, where its quaternary structure is critical for maintaining its full catalytic function. Hereditary fructose intolerance (HFI), a potentially fatal human autosomal recessive disease of carbohydrate metabolism, results with Aldolase B deficiency. Partially active Aldolase B, however, has been reported in an HFI individuals, which supports the hypothesis that adequate gluconeogenesis/glycolysis is maintained in HFI patients by the presence of partially active enzymes (1-4).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1000 \nImmunohistochemistry: 1:100-250 \nFlow Cytometry: 1:20 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A1335-10D

Size

100ul

Applications

FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Serum

Isotype

IgG

References

1. Rellos P, et al. J Biol Chem. 275(2): 1145-51, 2000. 2. Cross NC, et al. Cell. 53(6): 881-5, 1988 \n3. Brooks CC, et al. FASEB J. 8(1): 107-13, 1994. 4. Gregori C, et al. J Biol Chem, 273(39): 25237-25243, 1998

Additional Info

Recognizes human Adolase B. Species Crossreactivity: mouse, rat