Aldosterone EIA Kit

Aldosterone is a mineralocorticoid that controls sodium-potassium balance through the unidirectional salt reabsorption in a variety of tissues and glands. Synthesized from cholesterol in the zona glomerulosa of the adrenal cortex, secretion is regulated through the renin-angiotensin system. Angiotensin II and potassium stimulate primary secretion by increasing the rate of production of the steroid. Peripheral aldosterone levels are dependant on age and body position and in a normal upright adult aldosterone levels are typically less than 300 pg/mL. Aldosterone is typically secreted as the 18-glucuronide and the terahydro-3-glucuronide and this excretion is generally 2-20 ug/24 hour urine collection.Aldosterone measurement is useful in the investigation of primary aldosteronism (i.e., adrenal adenoma or carcinoma and adrenal cortical hyperplasia) and secondary aldosteronism (renovascular disease, salt depletion, potassium loading, cardiac failure with ascites, pregnancy, Bartter syndrome). The renin-angiotensin system is the primary regulator of the synthesis and secretion of aldosterone. Increased concentrations of potassium in the plasma may directly stimulate adrenal production of the hormone. Under physiologic conditions, pituitary adrenocorticotropic hormone is not a major factor in regulating aldosterone secretion.The B-Bridge Aldosterone Immunoassay kit is designed to quantitatively measure Aldosterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. Protocols are provided to prepare assay standards from 4,000 to 3.906 pg/mL or from 5,000 to 8.192 pg/mL. An aldosterone stock solution is provided to generate a standard curve. Samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. An aldosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to aldosterone to each well. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound aldosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected at 450nm.