Alexa Fluor(R) 488 anti-Histone H3 Phospho (Ser28)

H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-alpha (mixed linage kinase-like mitogen activated protein triple kinase alpha) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.

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SPECIFICATIONS

Catalog Number

641003

Size

25 tests

Applications

FC, ICC

Hosts

Rat

Reactivities

Hum

Clone

HTA28

Antigen

Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of Human Histone H3.

Gene Id

10919

Isotype

Rat IgG2a, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 microl per million cells or 5 microl per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 488 has a maximum emission of 519 nm when it is excited at 488 nm.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

Histone-H3

Regulatory Status

RUO