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Alexa Fluor(R) 488 anti-mouse I-Ek/rat RT1D

Alexa Fluor(R) 488 anti-mouse I-Ek/rat RT1D

Cat no: 110210

Supplier: BioLegend
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The 14-4-4S antibody reacts with the I-Ek MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2d, H-2p, and H-2r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-Ek.
Catalogue number: 110210
Reactivities: Mouse
Hosts: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
Size: 100 microg
Clone: 14-4-4S
Gene: 14969
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?0.25 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 488 has a maximum emission of 519 nm when it is excited at 488 nm.** Alexa Fluor(R) 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: C3H mouse skin graft and splenocytes
Isotype: Mouse (C3H.SW) IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: MHC Class II
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