Alexa Fluor(R) 488 anti-mouse TNF-alpha

TNF-alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-alpha. Monomeric mouse TNF-alpha is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

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SPECIFICATIONS

Catalog Number

506313

Size

100 microg

Applications

FC, ICC

Hosts

Rat

Reactivities

Mouse

Clone

MP6-XT22

Antigen

E. coli-expressed, recombinant mouse TNF-alpha

Gene Id

21926

Isotype

Rat IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?0.25 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 488 has a maximum emission of 519 nm when it is excited at 488 nm.** Alexa Fluor(R) 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

Tumor necrosis factor-alpha, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

Regulatory Status

RUO