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Alexa Fluor(R) 488 anti-rat CD25

Alexa Fluor(R) 488 anti-rat CD25

Cat no: 202108


Supplier: BioLegend
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CD25 is a 55 kD glycoprotein also known as IL-2 receptor alpha chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains alpha, beta, and gamma. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.
Catalogue number: 202108
Reactivities: Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 100 microg
Clone: OX-39
Gene: 16184
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?0.25 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 488 has a maximum emission of 519 nm when it is excited at 488 nm.** Alexa Fluor(R) 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: Rat T cell blasts from mixed lymphocyte reactions
Isotype: Mouse IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: IL-2Ra, IL-2 receptor chain a
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