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Alexa Fluor(R) 647 Annexin V

Alexa Fluor(R) 647 Annexin V

Cat no: 640911

Supplier: BioLegend
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Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells.Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution (cat. no. 420403/420404) or Propidium Iodide Solution (cat. no. 421301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.
Catalogue number: 640911
Applications: Flow Cytometry
Size: 25 tests
Gene: 308
Dilutions: Each lot of this product is quality control tested by staining of apoptotic cells and analyzing by flow cytometry. The suggested use of this reagent is 5 microl per 1X105 cells in a 100 microl volume of Annexin V Binding Buffer (cat. no. 422201). It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor(R) 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The Annexin V solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Annexin A5
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