Home  >  Products  >  Alexa Fluor(R) 647 anti-mouse CD272 (BTLA)
Alexa Fluor(R) 647 anti-mouse CD272 (BTLA)

Alexa Fluor(R) 647 anti-mouse CD272 (BTLA)

Cat no: 139106

Supplier: BioLegend
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, reduced effectiveness of T regulatory cells. BTLA takes an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effectors and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4. But further studies are needed to confirm. The existence of three distinct BTLA alleles was reported. The BTLA antibody reacts only with the C57BL/6 allele of BTLA but not the BALB/c allele of BTLA.
Catalogue number: 139106
Reactivities: Mouse
Applications: Flow Cytometry
Size: 100 microg
Clone: 6A6
Gene: 208154
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?0.25 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.** Alexa Fluor(R) 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: C57BL/6 BTLA Ig domain protein in CFA
Isotype: Armenian Hamster IgG
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: BTLA, B and T lymphocyte attenuator
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave