Alexa Fluor(R) 647 anti-mouse IL-21

IL-21 is a 17 kD member of the common gamma chain family of cytokines and mainly produced by CD4+ T subsets, including Th17, follicular helper T cells (TFH), Th2, memory CD4+ T cells, and NKT cells. IL-21 signals through a heterodimeric receptor complex containing IL-21R and the common cytokine receptor gamma chain (CD132). IL-21 has effects on T, B, NK, and myeloid cells, regulates both humoral and cell-mediated immunity. It has been reported that IL-21 is able to enhance the expansion of Th17 cells via the induction of IL-23R, cooperates with IL-7 or IL-15 to promote CD8+ T cell expansion. IL-21 is also known to regulate Ig production by driving the differentiation of B cells to antibody-producing plasma cells and involving in the development of follicular helper T cells (TFH). In addition, IL-21 exerts negative effect on lymphoid and myeloid cells, including induction of B cell apoptosis and inhibition of dendritic cell maturation and function. The type of actions mediated by IL-21 is presumably determined by its biological context, specific activation state of the target cells, as well as the cytokine milieu. IL-21/IL-21R system is involved in the pathogenesis of various autoimmune diseases and anti-tumor effects.

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SPECIFICATIONS

Catalog Number

516803

Size

25 microg

Applications

FC, ICC

Hosts

Rat

Reactivities

Mouse

Clone

BL25168

Gene Id

59067

Isotype

Rat IgG2b

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?0.25 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

Interleukin-21

Regulatory Status

RUO