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Alexa Fluor(R) 700 anti-mouse/human CD45R/B220

Alexa Fluor(R) 700 anti-mouse/human CD45R/B220

Cat no: 103231

Supplier: BioLegend
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CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.
Catalogue number: 103231
Reactivities: Human, Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 25 microg
Clone: RA3-6B2
Gene: 19264, 5788
Dilutions: This CD45R reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor(R) 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The CD45R antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: Abelson murine leukemia virus-induced pre-B tumor cells
Isotype: Rat IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: B220
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