ALK, phosphorylated (Tyr1078) (ALK, Anaplastic Lymphoma Kinase)

Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK- expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLC y, and PI3 kinase(1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLC y by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with cor\n-responding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase\ndomain of ALK (6-8). \n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137407

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9. 2. Iwahara, T. et al. (1997) Oncogene 14, 439-49. 3. Morris, S.W. et al. (1997) Oncogene 14, 2175-88. 4. Morris, S.W. et al. (1994) Science 263, 1281-4. 5. Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61. 6. Rikova, K. et al. (2007) Cell 131, 1190-203. 7. Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24. 8. Soda, M. et al. (2007) Nature 448, 561-6.

Additional Info

Recognizes endogenous levels of ALK when phosphorylated at Tyr1078.