Alkaline Phosphatase, Bovine (AP)

Alkaline Phosphatase is Dimeric protein having a molecular weight of 140kD, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity. Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.\n\nActivity: \n~1000U/mg (pH 9.6, 37 degrees C, with 0.025M glycine, 10% glycerol as buffer).\n\nStorage and Stability: \nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1355-65B

Size

1mg

Form

Supplied as a liquid in 5mM MgCl2, 0.1mM ZnCl2, 5mM TRIS, pH 7.0, 50% glycerol.

Purity

(same/more than) 95% pure by Gel Filtration.

References

1. Involvement of intestinal alkaline phosphatase in serum apolipoprotein B-48 level and its association with ABO and secretor blood group types. Biochem Biophys Res Commun 2006 Mar 3;341(1):33-8 2. Intestinal alkaline phosphatase gene expression is activated by ZBP-89. Am J Physiol Gastrointest Liver Physiol 2006 Apr;290(4):G737-46 3. Bovine intestinal alkaline phosphatase attenuates the inflammatory response in secondary peritonitis in mice. Infect Immun 2005 Jul;73(7):4309-14 4. Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase isozymes. FEBS J 2005 May;272(10):2477-86. 5. Differentiation-dependent activation of the human intestinal alkaline phosphatase promoter by HNF-4 in intestinal cells. Am J Physiol Gastrointest Liver Physiol 2005 Aug;289(2):G220-6 6. Differential regulation of intestinal alkaline phosphatase gene expression by Cdx1 and Cdx2. Am J Physiol Gastrointest Liver Physiol 2005 Aug;289(2):G285-90