Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA Kit

Cat no: ABIN415660

Supplier: Antibodies-online

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Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Liver/Bone/Kidney (ALPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

Catalogue number:	ABIN415660
Size:	96 tests
Accession:	P09242
Gene:	11647
Antigen:	ALPL
Sensitivity:	The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range:	0.156-10 ng/mL
Sample type:	Serum,Plasma,Tissue Homogenate,Biological Fluids
Entrez gene:	11647

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Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA Kit

Cat no: ABIN415660

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