Alkaline Phosphatase

Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). In vivo studies have identified CIAP as a potential therapeutic agent for the treatment of lipopolysaccharide (LPS)-mediated inflammation and sepsis, due to the ability of CIAP to neutralise and thereby detoxify (LPS).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilution: \nELISA: 1:1000 -1:10000\nOptimal dilutions to be determined by the researcher. \n\nRecommended Secondary Antibodies\nI1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4071-39

Size

1ml

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Prc

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

IgG

References

1. Braccia, A. et al. (2003) Microvillar membrane microdomains exist at physiological temperature. Role of galectin-4 as lipid raft stabilizer revealed by \"superrafts\". J. Biol. Chem. 278: 15679-15684.\n2. Beumer, C. et al. (2003) Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets. J. Pharmacol. Exp. Ther. 307: 737-744.

Additional Info

Recognizes calf intestinal alkaline phosphatase- (CIAP), a membrane-bound hydrolase enzyme highly expressed by intestinal mucosa. Species crossreactivity: porcine. Detects a band of ~67kD in porcine microvillar membranes.

Alternative Names

Intestinal Alkaline Phosphatase