Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
ELISA (Coating): 1:100-1:500
ELISA (Primary): 1:1000-1:30,000
Immunohistochemistry: 1:200-1:2000
Western Blot (Colorimetric): 1:1000-1:10,000
Western Blot (ECL): 1:1000-1:30,000
Immunocytochemistry: 1:200-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.