ALMS 1 (Alstrom Syndrome 1)

This protein contains a large tandem-repeat domain that contains no cysteine residues. Mutations in this gene have been associated with Alstrom syndrome. The encoded protein may play a role in hearing, sight, obesity, and liver function. Alternative splice variants have been described but their full length sequences have not been determined. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: > 1:32000\nWestern Blot: No signal obtained yet but low background observed in Human Muscle lysate at up to 0.5ug/ml.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1359

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype

IgG

References

General: Collin GB et al. Mutations in ALMS1 cause obesity, type 2 diabetes and neurosensory degeneration in AlstrГ¶m syndrome. Nat Genet 31:74-8 (2002).

Additional Info

Recognizes ALMS 1. The C-terminus of ALMS 1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not crossreact with this antibody. The N-terminus, not chosen as the peptide, is expected to cyclize during synthesis. Species Crossreactivity: Expected to crossreact with Human and Mouse due to sequence homology.