Home  >  Products  >  ALOX15 (Arachidonate 15-lipoxygenase, 15-Lipoxygenase 1, 15-Lipoxygenase Reticulocyte Arachidonate, 15-LOX, Arachidonate omega-6 Lipoxygenase)
ALOX15 (Arachidonate 15-lipoxygenase, 15-Lipoxygenase 1, 15-Lipoxygenase Reticulocyte Arachidonate, 15-LOX, Arachidonate omega-6 Lipoxygenase)

ALOX15 (Arachidonate 15-lipoxygenase, 15-Lipoxygenase 1, 15-Lipoxygenase Reticulocyte Arachidonate, 15-LOX, Arachidonate omega-6 Lipoxygenase)

Cat no: A1359-80C

Supplier: United States Biological
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ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1-5ug/ml using recombinant protein. The detection limit in sandwich ELISA is ~3ng/ml. Immunohistochemistry (Paraffin): 1.5ug/ml stained human small intestine tissue. Western Blot: 1-5ug/ml. Band detected at ~74.67kD using HeLa NE cell lysate. Band detected at ~98.93kD using immunogen protein lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A1359-80C
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2.
P type: Mab
Isotype: IgG2a,k
Purity: Purified.
References: 1.Selective survival rescue in 15-lipoxygenase-1 deficient retinal pigment epithelial cells by the novel docosahexaenoic acid-derived mediator, neuroprotectin D1. Calandria JM, Marcheselli VL, Mukherjee PK, Uddin J, Winkler JW, Petasis NA, Bazan NG.J Biol Chem. 2009 Jun 26;284(26):17877-82. Epub 2009 Apr 29.
Additional info: Recognizes human ALOX15.

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