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ALOX15 (Arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2)

ALOX15 (Arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2)

Cat no: A1359-80

Supplier: United States Biological
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Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:32,000. Western Blot: 0.2-0.5microg/ml. Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See A1359-80-P. Antibody
Catalogue number: A1359-80
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by affinity chromatography.
Alternative names: ALOX15; arachidonate 15-lipoxygenase; 15-LOX-1; 15-LOX2
References: McDuffie M, Maybee NA, Keller SR, Stevens BK, Garmey JC, Morris MA, Kropf E, Rival C, Ma K, Carter JD, Tersey SA, Nunemaker CS, Nadler JL. Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes. Diabetes. 2008 Jan;57(1):199-208. Epub 2007 Oct 16. PMID: 17940120
Additional info: Recognizes human ALOX15.

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