ALOX15 (Arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2)

Applications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nPeptide ELISA: 1:32,000. \nWestern Blot: 0.2-0.5microg/ml. Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\n\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n \n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See A1359-80-P. Antibody

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SPECIFICATIONS

Catalog Number

A1359-80

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

References

McDuffie M, Maybee NA, Keller SR, Stevens BK, Garmey JC, Morris MA, Kropf E, Rival C, Ma K, Carter JD, Tersey SA, Nunemaker CS, Nadler JL.\nNonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes.\nDiabetes. 2008 Jan;57(1):199-208. Epub 2007 Oct 16.\nPMID: 17940120

Additional Info

Recognizes human ALOX15.

Alternative Names

ALOX15; arachidonate 15-lipoxygenase; 15-LOX-1; 15-LOX2