Alpha Enolase, Recombinant Human, aa1-434 (Enolase 1)

Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography.\n\nRecombinant protein corresponding to human alpha-enolase was expressed in E. coli.\n\nAA sequence:\nMSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK\n\nSpecific Activity: \n~10 units/ml\n\nBiological Activity:\n1 unit will convert 1.0umole of 2-phosphoglycerate to phospho(enol)pyruvate per min at pH7.5 at 25C\n\n\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3222-77

Size

100ug

Reactivities

Hum

Form

Supplied as a liquid in 20 mM Tris-HCl, pH 7.5, 1mM MgSO4, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1.HYPERLINK \"http://www.ncbi.nlm.nih.gov/pubmed?term=%22Das%20R%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract\" Das R., et al. (2009) HYPERLINK \"javascript:AL_get(this,%20'jour',%20'Blood.');\" \\o \"Blood.\" Blood. 113(22):5371-2.\n2. Ueno NT., et al. (2008) Cancer Res. 68(22):9302-10.