alpha-Fetoprotein (AFP) ELISA Kit

Cat no: ABIN415390

Supplier: Antibodies-online

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alpha-Fetoprotein (AFP) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (aFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (aFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (aFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

Catalogue number:	ABIN415390
Size:	96 tests
Accession:	P02772
Gene:	11576
Antigen:	aFP
Sensitivity:	The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range:	1.563-100 pg/mL
Sample type:	Serum,Plasma,Biological Fluids
Entrez gene:	11576

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alpha-Fetoprotein (AFP) ELISA Kit

Cat no: ABIN415390

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