ALPL, ID (Alkaline Phosphatase, Tissue-nonspecific Isozyme, AP-TNAP, TNSALP, Alkaline Phosphatase Liver/Bone/Kidney Isozyme)

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nImmunohistochemistry: 1:10-1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4071-18C

Size

200ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Pab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgG

References

1. Panuccio,V., Am. J. Kidney Dis. 50(6), 1001-1008 (2007). 2. Brun-Heath,I., Eur J Med Genet 50(5), 367-378 (2007). 3. So,P.P., J. Rheumatol. 34(6), 1313-1322 (2007).

Additional Info

Recognizes human ALPL.