ALPP, NT (ALPP, PLAP, Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1) (Biotin)

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031817-Biotin

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Stec, B., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010) :\nWang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009)\nEstrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009)\nZhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009)\nRoberson, J.R., et al. Pediatr Blood Cancer 51(6):840-842(2008)

Additional Info

Human

Alternative Names

ALPP, PLAP, Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1