ALS2CR2 (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein, CALS-21, ILP-interacting protein, ILPIP, ILPIPA, MGC102916, PAPK, PRO1038, Pseudokinase ALS2CR2, Serine/threonine-protein kinase ALS2CR2, STE20-related kinase adaptor be

This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. \n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:32,000\nWestern Blot: 1-3ug/ml; ~35kD band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0kD according to NP_061041.2 (alpha isoform) and 31.2kD according to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48kD is observed. This band is not observed in the non-transfected HEK293.\nImmunohistochemistry: 3-5ug/ml in paraffin embedded Human Heart shows staining of myocardial fibres in transverse section.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1363-10H

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Sanna MG, da Silva Correia J, Luo Y, Chuang B, Paulson LM, Nguyen B, Deveraux QL, Ulevitch RJ. \nILPIP, a novel anti-apoptotic protein that enhances XIAP-mediated activation of JNK1 and protection against apoptosis. J Biol Chem. 2002 Aug 23;277(34):30454-62

Additional Info

Recognizes human ALS2CR2. Species sequence homology: canine and bovine.\nExpected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).

Alternative Names

pseudokinase ALS2CR2, STRAD beta, STE20-related kinase adaptor beta, ILP-interacting protein ILPIPA, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, PRO1038, CALS-21, ILPIPA, PAPK, ILPIP, ALS2CR2, STRADB