AMD1 (SAMDC , S-adenosylmethionine Decarboxylase Proenzyme, AdoMetDC, S-adenosylmethionine Decarboxylase alpha Chain, S-adenosylmethionine Decarboxylase beta Chain, AMD1, AMD)

S-adenosylmethionine decarboxylase (AdoMet-DC), also known as S-adenosylmethionine decarboxylase proenzyme (SAMDC), is a key enzyme in polyamine biosynthesis. It is localized to chromosome region 6q21-q22. SAMDC has an unusual distribution in polysomes from cells of T lymphocyte origin. It associates predominantly with monosomes and small polysomes with none located in the preribosomal or ribonucleoprotein pool. SAMDC is a critical regulatory enzyme of the polyamine synthetic pathway, and a well-studied drug target. Since SAMDC is a key regulatory enzyme in the synthesis of spermidine and spermine, the marked increase in SAMDC activity in the neonate and the sustained high enzyme levels throughout adulthood, imply a role for these polyamines in both development and mature brain function.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.1-0.5ug/ml \nImmunohistochemistry (Paraffin): 0.5-1ug/ml. Antigen retrieval by heat is required.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1372-24C

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Maric, SC., et al., Cytogenet Cell Genet. 1995; 70(3-4): 195-9. 2. Hill, JR., Morris, DR., J Biol Chem. 1992; 267(30): 21886-93. 3. Ekstrom, JL, et al., Structure. 1999; 7(5): 583-95. 4. Morrison, LD., Becker, L., Kish, SJ., Brain Res Dev Brain Res. 1993; 73(2): 237-41.

Additional Info

Recognizes human AMD1. Species Crossreactivity: mouse and rat