AMICA is a 60kD type I transmembrane glycoprotein member of the JAM family of adhesion molecules. Human AMICA has an extracellular domain (ECD) of 275aa and a cytoplasmic tail of 98aa. The ECD contains two Ig-like regions. There is an N-terminal C2-type Ig-like domain and a membrane proximal V-type Ig-like domain. AMICA is expressed by leukocytes and is concentrated at areas of cell-cell contact. It forms noncovalent homodimers on the cell surface and binds CXADR on epithelial cells. Two splice variants apparently exist that only involve the first 40aa of the precursor. Human AMICA ECD is 62% and 76% aa identical to mouse and dog AMICA ECD, respectively.
Applications:
Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume (same/less than) 200ul. Tested using blood-derived monocytes.
Direct ELISA: 0.5-1.0ug/ml The detection limit is ~2ng/well.
Western Blot: 1-2ug/ml Using a colorimetric detection system. the detection limit is ~25ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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