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AMICA (Adhesion Molecule that Interacts with CXADR Antigen 1, Junction Adhesion Molecule-Like, JAM-L, JAML)

Cat no: A1372-37

AMICA (Adhesion Molecule that Interacts with CXADR Antigen 1, Junction Adhesion Molecule-Like, JAM-L, JAML)

AMICA (Adhesion Molecule that interacts with CXADR antigen 1), also known as junction adhesion molecule like (JAM-L) is a 60kD type I transmembrane glycoprotein member of the JAM family of adhesion molecules. Human AMICA has an extracellular domain (ECD) of 275 amino acids (aa) and a cytoplasmic tail of 98 amino acids. The ECD contains two Ig-like regions. There is an N-terminal C2-type Ig-like domain and a membrane proximal V-type Ig-like domain. AMICA is expressed by leukocytes and is concentrated at areas of cell-cell contact. It forms noncovalent homodimers on the cell surface and binds CXADR on epithelial cells. Two splice variants apparently exist that only involve the first 40 aa of the precursor. Human AMICA ECD is 62% and 76% aa identical to mouse and dog AMICA ECD, respectively.\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nELISA: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1372-37

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Recognizes human AMICA.

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