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AMIGO3 (Amphoterin-Induced Gene and ORF 3, Alivin-3)

Cat no: A1372-59

AMIGO3 (Amphoterin-Induced Gene and ORF 3, Alivin-3)

AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively.\n\nApplications: \nSuitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nDirect ELISA: 0.5- 1.0ug/ml detection limit for rmAMIGO3 is ~ 1ng/well.\nWestern Blot: 1-2ug/ml detection limit for rmAMIGO3 is ~ 25ng/lane under non-reducing and reducing conditions.\nImmunohistochemistry: 25ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1372-59

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder in 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes mouse AMIGO3.

Read more on Supplier website

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info
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