AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively.
Applications:
Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5- 1.0ug/ml detection limit for rmAMIGO3 is ~ 1ng/well.
Western Blot: 1-2ug/ml detection limit for rmAMIGO3 is ~ 25ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 25ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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